Efficient Protocol of Micropropagation, and Organogenesis of Euphorbia pulcherrima Willd. Plants via Stem and Leaf Segments

Gharbia H. Danial, Diaa A. Ibrahim

Euphorbia pulcherrima, Micropropagation, shoot tips, Organogenesis.

A highly efficient plant propagation protocol was developed for Euphorbia pulcherrima Willd. using nodal shoot segments. Different concentrations of 6-Benzylaminopurine (BAP) singly or in combination with both GA3 and adenine sulfate. Use of BAP at 0.5 mg/l combination with 20 mg/l adenine sulfate induced the highest frequency (100%) of shoot induction as well as maximum number of shoots per explant (10.6) followed by using the combination of 1.5 mg/l BAP with 0.5 mg/l GA3 which create 6.76 shoot /explants, while lower number of shoot/explants (5.88) was recorded in MS medium supplemented with 0.5 mg/l BAP and the proliferated shoot clumps were further multiplied and elongated. The highest rooting frequency (77.8%) as well as highest number of roots (4.9) was observed in full strength MS medium supplemented with 1.0 mg/l Indole-acetic acid (IAA). In order to study the effects of organs culture (stem and leaf) in vitro culture of poinsettia, shoot regeneration frequently was 100% when the stem explants were placed on MS medium containing 1.0 mg/l BAP combination with 0.2 mg/l of NAA. Highest number of shoots per explant (6.95) was recorded followed by (5.83) shoots per explants in MS medium containing BAP 0.5 mg/l combination with GA3 0.1 mg/l. Shoot regeneration from Leaf explants reached 77.73% when the leaf explants were placed on MS medium containing BAP 1.0 mg/l combination with 0.2 mg/l of IAA and highest number of shoots per explant (3.00) was recorded. Regenerated plantlets were acclimatized successfully in the green house and finally transplanted in the pots.

10.22161/ijaers.3.8.8